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Expression of glial fibrillary acidic protein (GFAP; green) and a microglia-specific ionized calcium binding adapter protein (Iba1, red) in the rodent brain. GFAP is a classical marker for astrocytes and oligodendrocytes. Ca2+ is an important regulator of neural function and its homeostasis depends on numerous calcium-binding proteins. In addition to being a marker for microglia (no colocalization with GFAP), Iba1 is an indicator of glia-dependent regulation of Ca2+ dynamics in neural plasticity.
Chuma Okere, Ph.D., Waterhouse Laboratory