Techniques

The laboratory utilizes a wide variety of techniques to investigate the role of neuropeptides in reinforcing substance intake. These include:

Quantitative real-time PCR

ELISA, immunohistochemistry, in situ hybridization

Operant and non-operant (voluntary) substance self-administration

Activity chamber locomotor behavior, hole board exploration, place preference, elevated plus maze anxiety, and light-dark box anxiety

Microinjection, tract tracing, and chemogenetics