Microanatomy Mid-Block Practical
Study Hints and Structure Checklist
The following are some study hints, and a list of structures that you should be prepared to identify for your practical exam. Keep in mind that some questions may ask about the function, location or composition of these structures, so you need to be prepared beyond simply recognizing these structures.
Things
to keep in mind when studying for the exam:
-
Be sure you understand
what we mean when we ask you to 'classify' a structure vs. 'identify'
a structure
We make a fuss about classifying vs. identifying structures mainly
when we are talking about epithelium, glands and connective tissues,
so later in the course this will not be such an issue. For now, however,
you should realize that to classify a structure means to place it
in a group with similar structures, while to identify it means to
give its own unique name - the name that distinguishes it from all
other similar structures.
Suppose we took you as an example, and wanted to classify vs. identify
you. You are a male of the species Homo sapiens. That would be classifying
you. You are also Michael Carrington. That is identifying you.
To take an example from histology, suppose we are looking at the epithelium
that lines a blood vessel. We classify it as a simple squamous epithelium.
We identify it by saying that it is the endothelium. This name distinguishes
it from all other simple squamous epithelia in the body.
- When we say 'be
specific' we mean it:
For many of the organs
that you have learned about, you are often required to be able to
distinguish not only what organ it is, but where in the organ the
section comes from, or from what physiological state it is in. For example:
- Mammary
Gland: When
looking at a slide, you need to be able to distinguish whether the glands
are inactive, proliferating(pregnancy) or lactating.
- Don't forget to
utilize all the resources available on the website and in the laboratory:
- For each laboratory, you
can find 'focus questions' that may help clarify details about the histological
structure or function of cells, tissues or organs that you need to identify.
From the main webpage, click on a case, select the laboratory assignment
and scroll to the end of the assignment. Test yourself - think about
what the answer is, then check it by clicking on the 'answer' link.
- The online lab-manuals contain both annotated micrograph images and virtual slides (many with annotations) that can help you learn how to locate and identify particular structures.
- Use the histology atlas
online (link is on the main webpage), or videodisks in the laboratory.
These are particularly useful for learning the cell types and details
of tissue and organ structure when higher magnification views are useful.
Using the macintosh computer attached to the videodisk player (one set
up in each lab) you can use a quiz mode to test yourself. Information
on how to use this feature can be found in the course
information section of the microanatomy website.
- Don't forget to look at
the electron micrographs - in the Rhodin atlas in your locker, or online
(link on main page). Ross also has some good EM images.
- Study from your glass and virtual slide
collections. Quiz each
other by displaying a slide and having other members of your group guess
what slide is being shown - just cover up the labels.
- The "Structure Checklists" at the end of each laboratory can be used as a guide for deciding which structures to focus on when you are studying.
- A useful tip:
When you are studying,
keep in mind a couple of key features that can help you quickly identify
a particular tissue or an organ, particularly those features that
are unique to that organ, region or tissue that can help you distinguish
it from similar ones.
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Structure Checklist:
Microscopy, Stains and Organelles (based on your microscopy and cell structure self-study guides)
LIGHT MICROSCOPY
Cytoplasm vs nucleus vs nucleolus
Heterochromatic vs euchromatic nucleus
Nissl Body
Collagen in a trichrome stain
Glycogen in Best's carmine stain
Any PAS-positive structure
Reticular fibers in a silver stain
Elastic fibers stained with aldehyde-fuchsin
Myelin stained with osmium |
ELECTRON MICROSCOPY
You should be able to recognize cellular organelles (e.g. lipid droplets, lysosomes, rough endoplasmic reticulum) and cytoskeletal structures in order to be able to identify the primary function of a differentiated cell. See examples in the 'differentiated cell' chapter of your cell structure self study.
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Epithelia and Glands (based on your epithelium self-study guide)
LIGHT MICROSCOPY
Simple squamous epithelium
Endothelium
Mesothelium
Simple cuboidal epithelium
Simple columnar epithelium
Goblet cell
Striated (brush) border
Maximally keratinized stratified squamous epithelium
Minimally keratinized stratified squamous epithelium
Stratified columnar epithelium
Pseudostratified Columnar epithelium with Cilia (Respiratory epithelium)
Transitional epithelium
Terminal Bar
Desmosomes (spines of stratum spinosum)
Serous gland cells
Mucous gland cells
Myoepithelial cells
Sebaceous gland
Eccrine and apocrine sweat glands |
ELECTRON MICROSCOPY
Epithelial types (as listed at left)
Junctional complex (and its components)
Cilia
Basal bodies
Microvilli
Terminal web
Stereocilia
Goblet cells
Glycocalyx
Basal Lamina
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Integument (skin and appendages)
LIGHT MICROSCOPY
Thick vs. thin skin
Epidermis vs. dermis
Keratinocyte (including characteristic features in each layer, e.g. spines (desmosomes) in stratum spinosum; keratohyalin granules in stratum granulosum)
Layers of the epidermis (basale, spinosum, etc..)
Melanin
Melanocyte
Reticular vs papillary layer of dermis
Sebaceous gland
Apocrine sweat gland
Eccrine sweat gland (secretory vs duct portions) |
ELECTRON MICROSCOPY
Keratinocyte
Layers of the epidermis
Melanocyte
Langerhans cell
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Connective tissue (based on your laboratory assignment and CT cells self-study guide)
LIGHT MICROSCOPY
Fibroblast
Collagen fibers
Lamina propria
Elastic fibers
Elastic lamellae
Reticular fibers
White fat
Brown fat
Macrophage
Mast cell
Lymphocyte
Plasma cell
Eosinophil
Loose connective tissue
Loose cellular connective tissue
Dense irregular connective tissue
Dense regular connective tissue
Mucous connective tissue |
ELECTRON MICROSCOPY
Collagen fibrils
Collagen fibers
Elastic fibers
Reticular fibers
Fibroblast
Macrophage
Plasma Cell
Mast Cell
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Mammary Gland
LIGHT MICROSCOPY
Mammary Gland (inactive, proliferating vs lactating) |
ELECTRON MICROSCOPY
Apocrine secretion of mammary glands
Merocrine secretion of mammary glands
Myoepithelial cells
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Muscle
LIGHT MICROSCOPY
Skeletal vs cardiac vs smooth muscle in cross and longitudinal section
Sarcomere
A band
I band
Endomysium vs perimysium vs epimysium
Intercalated Disk |
ELECTRON MICROSCOPY
Thick filaments
Thin filaments
I band
A band
Z line
H band
Sarcoplasmic reticulum
T Tubule
Triad of skeletal muscle
Myoneural junction of skeletal muscle
Intercalated disc
Dense bodies of smooth muscle
Caveolae of smooth muscle
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Nerve
LIGHT MICROSCOPY
Dorsal root (spinal) ganglion
Pseudounipolar neuron
Satellite cells
Schwann cell
Autonomic Ganglion
Myelin
Alpha motor neuron
Nissl bodies
Axon hillock
Myenteric plexus
Submucosal plexus
Peripheral nerve in xs and ls
Endoneurium vs perineurium vs epineurium
Pacinian corpuscle
Meissner's corpuscle
Muscle spindle |
ELECTRON MICROSCOPY
Synapse
Synaptic vesicles
Myelin sheath
Node of Ranvier
Cleft of Schmidt-Lanterman
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Cartilage and Bone
LIGHT MICROSCOPY
Osteon
Haversian Canal
Volkmann's Canal
Lacuna
Canaliculus
Interstitial lamellae
Cement Lines
Circumferential Lamellae
Periosteum
Endosteum
Osteoblast
Osteocyte
Osteoclast
Howship's lacuna
Woven Bone vs Lamellar Bone
Cancellous Bone vs Cortical Bone
Hyaline vs Elastic vs Fibrocartilage
Chondrocyte
Lacuna
Territorial vs Interterrorial matrix
Isogenous group
Perichondrium
Symphysis
Synovial membrane
Articular cartilage |
ELECTRON MICROSCOPY
Osteocyte
Osteoblast
Osteoclast
Lacuna
Chondrocyte
Chondroblast
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Bone Formation and Remodeling
LIGHT MICROSCOPY
Intramembranous vs endochondral bone formation
Zones of the growth plate
Epiphysis vs diaphysis
Bone spicules with a calcified cartilage core (mixed spicules)
Evidence of remodeling vs modeling |
ELECTRON MICROSCOPY
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Respiratory System
LIGHT MICROSCOPY
Respiratory epithelium
Olfactory epithelium
Bowman's Glands
Larynx
False vs true vocal folds
Trachea
Intrapulmonary Bronchus
Conducting bronchiole
Terminal bronchiole
Respiratory bronchiole
Alveolar duct
Alveolus
Alvelolar macrophage
Alveolar capillaries
Pulmonary artery branches in lung
Pulmonary vein branches in lung
Mesothelium |
ELECTRON MICROSCOPY
Type I Pneumocyte
Type II Pneumocyte
minimal air-blood barrier
Alveolar macrophage
Alveolar capillary
Olfactory epithelium and its cells
Bronchiolar epithelium
Respiratory epithelium
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Cardiovascular System
LIGHT MICROSCOPY
Tunica intima, media vs adventitia
Muscular artery
Internal elastic lamina
External elastic lamina
Elastic artery
Fenestrated elastic membrane
Arteriole
Capillary
Sinusoidal capillary
Endothelium
Pericyte
Vein vs. venule
Large lymphatic vessel
Endocardium vs myocardium vs epicardium
AV valve
Chordae tendinae
Papillary muscle
Trabeculae carnae
Pectinate muscle
Annulus fibrosis
Purkinje fibers
SA node
Ventricle vs. Atrium
Right side of heart vs. left |
ELECTRON MICROSCOPY
Continuous vs fenestrated capillaries (w and w/out diaphragms)
Sinusoids
Endothelium
Pericyte
Intercalated disk
Fascia adherens
Purkinje fiber
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