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Microanatomy Mid-Block Practical

Study Hints and Structure Checklist

The following are some study hints, and a list of structures that you should be prepared to identify for your practical exam. Keep in mind that some questions may ask about the function, location or composition of these structures, so you need to be prepared beyond simply recognizing these structures.

Things to keep in mind when studying for the exam:

  1. Be sure you understand what we mean when we ask you to 'classify' a structure vs. 'identify' a structure
    We make a fuss about classifying vs. identifying structures mainly when we are talking about epithelium, glands and connective tissues, so later in the course this will not be such an issue. For now, however, you should realize that to classify a structure means to place it in a group with similar structures, while to identify it means to give its own unique name - the name that distinguishes it from all other similar structures.
    Suppose we took you as an example, and wanted to classify vs. identify you. You are a male of the species Homo sapiens. That would be classifying you. You are also Michael Carrington. That is identifying you.
    To take an example from histology, suppose we are looking at the epithelium that lines a blood vessel. We classify it as a simple squamous epithelium. We identify it by saying that it is the endothelium. This name distinguishes it from all other simple squamous epithelia in the body.

  2. When we say 'be specific' we mean it:

    For many of the organs that you have learned about, you are often required to be able to distinguish not only what organ it is, but where in the organ the section comes from, or from what physiological state it is in. For example:

  • Mammary Gland: When looking at a slide, you need to be able to distinguish whether the glands are inactive, proliferating(pregnancy) or lactating.
  1. Don't forget to utilize all the resources available on the website and in the laboratory:
  • For each laboratory, you can find 'focus questions' that may help clarify details about the histological structure or function of cells, tissues or organs that you need to identify. From the main webpage, click on a case, select the laboratory assignment and scroll to the end of the assignment. Test yourself - think about what the answer is, then check it by clicking on the 'answer' link.
  • The online lab-manuals contain both annotated micrograph images and virtual slides (many with annotations) that can help you learn how to locate and identify particular structures.
  • Use the histology atlas online (link is on the main webpage), or videodisks in the laboratory. These are particularly useful for learning the cell types and details of tissue and organ structure when higher magnification views are useful. Using the macintosh computer attached to the videodisk player (one set up in each lab) you can use a quiz mode to test yourself. Information on how to use this feature can be found in the course information section of the microanatomy website.
  • Don't forget to look at the electron micrographs - in the Rhodin atlas in your locker, or online (link on main page). Ross also has some good EM images.
  • Study from your glass and virtual slide collections. Quiz each other by displaying a slide and having other members of your group guess what slide is being shown - just cover up the labels.
  • The "Structure Checklists" at the end of each laboratory can be used as a guide for deciding which structures to focus on when you are studying.
  1. A useful tip:

    When you are studying, keep in mind a couple of key features that can help you quickly identify a particular tissue or an organ, particularly those features that are unique to that organ, region or tissue that can help you distinguish it from similar ones.

 

 

 

Structure Checklist:

Microscopy, Stains and Organelles (based on your microscopy and cell structure self-study guides)

LIGHT MICROSCOPY

Cytoplasm vs nucleus vs nucleolus

Heterochromatic vs euchromatic nucleus

Nissl Body

Collagen in a trichrome stain

Glycogen in Best's carmine stain

Any PAS-positive structure

Reticular fibers in a silver stain

Elastic fibers stained with aldehyde-fuchsin

Myelin stained with osmium

ELECTRON MICROSCOPY

You should be able to recognize cellular organelles (e.g. lipid droplets, lysosomes, rough endoplasmic reticulum) and cytoskeletal structures in order to be able to identify the primary function of a differentiated cell. See examples in the 'differentiated cell' chapter of your cell structure self study.

 

 

 

 

Epithelia and Glands (based on your epithelium self-study guide)

LIGHT MICROSCOPY

Simple squamous epithelium

Endothelium

Mesothelium

Simple cuboidal epithelium

Simple columnar epithelium

Goblet cell

Striated (brush) border

Maximally keratinized stratified squamous epithelium

Minimally keratinized stratified squamous epithelium

Stratified columnar epithelium

Pseudostratified Columnar epithelium with Cilia (Respiratory epithelium)

Transitional epithelium

Terminal Bar

Desmosomes (spines of stratum spinosum)

Serous gland cells

Mucous gland cells

Myoepithelial cells

Sebaceous gland

Eccrine and apocrine sweat glands

ELECTRON MICROSCOPY

Epithelial types (as listed at left)

Junctional complex (and its components)

Cilia

Basal bodies

Microvilli

Terminal web

Stereocilia

Goblet cells

Glycocalyx

Basal Lamina

 

 

 

 

Integument (skin and appendages)

LIGHT MICROSCOPY

Thick vs. thin skin

Epidermis vs. dermis

Keratinocyte (including characteristic features in each layer, e.g. spines (desmosomes) in stratum spinosum; keratohyalin granules in stratum granulosum)

Layers of the epidermis (basale, spinosum, etc..)

Melanin

Melanocyte

Reticular vs papillary layer of dermis

Sebaceous gland

Apocrine sweat gland

Eccrine sweat gland (secretory vs duct portions)

ELECTRON MICROSCOPY

Keratinocyte

Layers of the epidermis

Melanocyte

Langerhans cell

 

 

 

 

 

Connective tissue (based on your laboratory assignment and CT cells self-study guide)

LIGHT MICROSCOPY

Fibroblast

Collagen fibers

Lamina propria

Elastic fibers

Elastic lamellae

Reticular fibers

White fat

Brown fat

Macrophage

Mast cell

Lymphocyte

Plasma cell

Eosinophil

Loose connective tissue

Loose cellular connective tissue

Dense irregular connective tissue

Dense regular connective tissue

Mucous connective tissue

ELECTRON MICROSCOPY

Collagen fibrils

Collagen fibers

Elastic fibers

Reticular fibers

Fibroblast

Macrophage

Plasma Cell

Mast Cell

 

 

 

 

 

 

Mammary Gland

LIGHT MICROSCOPY

Mammary Gland (inactive, proliferating vs lactating)

ELECTRON MICROSCOPY

Apocrine secretion of mammary glands

Merocrine secretion of mammary glands

Myoepithelial cells

 

 

Muscle

LIGHT MICROSCOPY

Skeletal vs cardiac vs smooth muscle in cross and longitudinal section

Sarcomere

A band

I band

Endomysium vs perimysium vs epimysium

Intercalated Disk

ELECTRON MICROSCOPY

Thick filaments

Thin filaments

I band

A band

Z line

H band

Sarcoplasmic reticulum

T Tubule

Triad of skeletal muscle

Myoneural junction of skeletal muscle

Intercalated disc

Dense bodies of smooth muscle

Caveolae of smooth muscle

 

 

 

Nerve

LIGHT MICROSCOPY

Dorsal root (spinal) ganglion

Pseudounipolar neuron

Satellite cells

Schwann cell

Autonomic Ganglion

Myelin

Alpha motor neuron

Nissl bodies

Axon hillock

Myenteric plexus

Submucosal plexus

Peripheral nerve in xs and ls

Endoneurium vs perineurium vs epineurium

Pacinian corpuscle

Meissner's corpuscle

Muscle spindle

ELECTRON MICROSCOPY

Synapse

Synaptic vesicles

Myelin sheath

Node of Ranvier

Cleft of Schmidt-Lanterman

 

 

Cartilage and Bone

LIGHT MICROSCOPY

Osteon

Haversian Canal

Volkmann's Canal

Lacuna

Canaliculus

Interstitial lamellae

Cement Lines

Circumferential Lamellae

Periosteum

Endosteum

Osteoblast

Osteocyte

Osteoclast

Howship's lacuna

Woven Bone vs Lamellar Bone

Cancellous Bone vs Cortical Bone

Hyaline vs Elastic vs Fibrocartilage

Chondrocyte

Lacuna

Territorial vs Interterrorial matrix

Isogenous group

Perichondrium

Symphysis

Synovial membrane

Articular cartilage

ELECTRON MICROSCOPY

Osteocyte

Osteoblast

Osteoclast

Lacuna

Chondrocyte

Chondroblast

 

 

 

 

Bone Formation and Remodeling

LIGHT MICROSCOPY

Intramembranous vs endochondral bone formation

Zones of the growth plate

Epiphysis vs diaphysis

Bone spicules with a calcified cartilage core (mixed spicules)

Evidence of remodeling vs modeling

ELECTRON MICROSCOPY

 

 

 

 

Respiratory System

LIGHT MICROSCOPY

Respiratory epithelium

Olfactory epithelium

Bowman's Glands

Larynx

False vs true vocal folds

Trachea

Intrapulmonary Bronchus

Conducting bronchiole

Terminal bronchiole

Respiratory bronchiole

Alveolar duct

Alveolus

Alvelolar macrophage

Alveolar capillaries

Pulmonary artery branches in lung

Pulmonary vein branches in lung

Mesothelium

ELECTRON MICROSCOPY

Type I Pneumocyte

Type II Pneumocyte

minimal air-blood barrier

Alveolar macrophage

Alveolar capillary

Olfactory epithelium and its cells

Bronchiolar epithelium

Respiratory epithelium

 

 

 

 

Cardiovascular System

LIGHT MICROSCOPY

Tunica intima, media vs adventitia

Muscular artery

Internal elastic lamina

External elastic lamina

Elastic artery

Fenestrated elastic membrane

Arteriole

Capillary

Sinusoidal capillary

Endothelium

Pericyte

Vein vs. venule

Large lymphatic vessel

Endocardium vs myocardium vs epicardium

AV valve

Chordae tendinae

Papillary muscle

Trabeculae carnae

Pectinate muscle

Annulus fibrosis

Purkinje fibers

SA node

Ventricle vs. Atrium

Right side of heart vs. left

ELECTRON MICROSCOPY

Continuous vs fenestrated capillaries (w and w/out diaphragms)

Sinusoids

Endothelium

Pericyte

Intercalated disk

Fascia adherens

Purkinje fiber